描述：THE™ His Tag Antibody, mAb, Mouse （subtype IgG1） has very high affinity. Tests performed at GenScript show that the antibody can also recognize 4xHis- and 5xHis-tags.
| 品牌 | GenScript/金斯瑞 | 貨號 | A00186 |
|---|---|---|---|
| 規(guī)格 | 100ug | 供貨周期 | 一周 |
| 應(yīng)用領(lǐng)域 | 生物產(chǎn)業(yè) |
THE™ 代表了"THE unmistakable, THE irreplaceable, THE best quality"。金斯瑞選擇穩(wěn)定、高產(chǎn)的雜交瘤細胞克隆，然后使用相應(yīng)的抗原進行篩選分離，制備出特異性高、親和力強的THE™ 系列金牌抗體。
您在實驗中是否曾經(jīng)因為樣品量小、標(biāo)簽只有部分暴露、融合蛋白變性或其它棘手的問題而感到煩惱？金斯瑞THE™ 系列金牌抗體，讓您的這些煩惱迎刃而解！
同時，我們也為您提供其它優(yōu)質(zhì)的免疫學(xué)相關(guān)產(chǎn)品。如憑借**的多肽合成能力，我們能夠提供大多數(shù)抗體的封閉肽，讓您確認觀察到的信號是由抗體與靶抗原之間的相互作用產(chǎn)生，而不是由于非特異性結(jié)合而產(chǎn)生。
靈敏度—比市場上其他暢銷的抗體更靈敏；
特異性—無交叉反應(yīng)；
親和力—-低解離常數(shù)Kd；
識別能力—能夠識別并結(jié)合廣泛的物種及表達系統(tǒng)的靶抗原；
低背景—無其它反應(yīng)背景；
便捷—適用范圍廣，產(chǎn)品全面；
穩(wěn)定—-好的批次間一致性，凍干狀態(tài)下，可至少穩(wěn)定保存2年。
Description | Monoclonal antibodies specific to six histidine tags can greatly improve the effectiveness of several different kinds of immunoassays, helping researchers identify, detect, and purify polyhistidine fusion proteins in bacteria, insect cells, and mammalian cells. However, since 6XHis-tag is poorly immunogenic, it needs to be conjugated to KLH or some other carrier as an immunogen. After hundreds of selection cycles, researchers at GenScript successfully isolated an antibody against His-tag. THE™ His Tag Antibody, mAb, Mouse (subtype IgG1) has very high affinity. Tests performed at GenScript show that the antibody can also recognize 4xHis- and 5xHis-tags. This means that even if the 6xHis-tag is only partially exposed, it will still be recognized and bound by this antibody. THETM His Tag mAb is produced from mice ascites and purified by protein A affinity column. This antibody recognizes native as well as denatured forms of synthetic polyhistidine and polyhistidine-tagged fusion proteins. The product reacts with fusion proteins expressed in bacteria, insect cells, and mammalian cells. THETM His Tag mAb recognizes His tags placed at N-terminal, C-terminal, and internal regions of fusion proteins. THETM His Tag mAb can be used in Western blot analyses, Dot blot analyses, ELISA, immunofluorescent staining, and flow cytometry of cultured cells. |
| Host Species | Mouse |
| Conjugation | Unconjugated |
| Immunogen | A synthetic peptide HHHHHH coupled to KLH |
| Fusion Partner | Spleen cells were fused with SP2/0-Ag14 mouse myeloma cells |
| Purification | Protein A affinity column |
| Subclass | IgG1, k |
| Clone ID | 6G2A9 |
| Concentration | 0.5 mg/ml, lyophilized with PBS, pH 7.4, containing 0.02% sodium azide |
| Reconstitution | Reconstitute the lyophilized antibody with deionized water (or equivalent) to a final concentration of 0.5 mg/ml. |
| Specificity | THE™ His Tag Antibody, mAb, Mouse recognizes C-terminal, N-terminal, and internal His tagged fusion proteins. |
| Storage | The antibody is stable in lyophilized form if stored at -20°C or below. The reconstituted antibody can be stored for 2-3 weeks at 2-8°C. For long term storage, aliquot and store at -20°C or below. Avoid repeated freezing and thawing cycles. |
| Note | GenScript can offer this product according to your requirement, including product size, buffer components,etc. |
| Working concentrations for specific applications should be determined by the investigator. The appropriate concentrations may be affected by secondary antibody affinity, antigen concentration, the sensitivity of the method of detection, temperature, the length of the incubations, and other factors. The suitability of this antibody for applications other than those listed below has not been determined. The following concentration ranges are recommended starting points for this product. ELISA: 0.05-0.2 µg/ml Western Blot: 0.1-0.2 µg/ml Immunoprecipitation (IP): 1 µg/ml Immunofluorescent staining: 1 µg/ml Flow cytometry (FACS): 1 µg/ml TR-FRET assays: user-optimized Other applications: user-optimized |
公司介紹：
漠寶（廈門）生物科技有限公司是一家致力于生命科學(xué)和生物技術(shù)領(lǐng)域的高科技企業(yè)，依托于廈門大學(xué)人才力量。公司主要專注于細胞生物學(xué)與蛋白質(zhì)分析相關(guān)產(chǎn)品的開發(fā)與銷售。公司主要代理和自產(chǎn)國產(chǎn)優(yōu)質(zhì)試劑和設(shè)備，以期為生命科學(xué)多研究領(lǐng)域的科研人員提供美而精的產(chǎn)品。我們相信經(jīng)過幾年的努力，漠寶生物一定能成為細胞生物學(xué)、免疫學(xué)等領(lǐng)域優(yōu)質(zhì)的供應(yīng)商，促進國產(chǎn)試劑替代進口試劑的發(fā)展，加速國產(chǎn)關(guān)鍵性原料研發(fā)進程，減輕困擾我們的進口試劑和設(shè)備依賴病。
漠寶生物將以更好的客戶服務(wù)、完備的產(chǎn)品供應(yīng)、深厚的專業(yè)知識和優(yōu)秀的人脈資源，在同行業(yè)中保持良好的企業(yè)形象和競爭優(yōu)勢。我們將不斷引進和開發(fā)優(yōu)質(zhì)的產(chǎn)品，滿足廣大客戶的需求，在世界各國大力發(fā)展生命科學(xué)的時代背景下，我們將以為“中國生命科學(xué)發(fā)展而盡上一份綿薄之力"為使命，為科學(xué)人員創(chuàng)造的科研成果提供強有力的支撐。
公司將不斷豐富和借助于優(yōu)秀人才和技術(shù)優(yōu)勢，致力于研發(fā)、生產(chǎn)出品質(zhì)優(yōu)異，行業(yè)前茅的細胞生物學(xué)與蛋白質(zhì)分析產(chǎn)品，力爭在不久的將來將漠寶打造為生命科學(xué)領(lǐng)域的優(yōu)秀品牌。
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